abstract：:While conducting our research on mutations in the human blood platelet glycoprotein Ib-alpha (GPIbalpha) gene, we detected an unusual deletion of 84 bp. This deletion took place in vitro, during PCR and between two direct repeats. It was observed that the deletion could be detected either by the direct sequencing of t...

journal_title：Molecular biotechnology

authors： Hadj Kacem B,Gargouri J,Gargouri A

更新日期：2008-09-01 00:00:00

abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...

journal_title：Molecular biotechnology

authors： Lefranc MP

更新日期：2008-09-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:RNA interference methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in delivering small interfering RNA (siRNA) into mesenchymal stem ce...

journal_title：Molecular biotechnology

authors： Zhao M,Yang H,Jiang X,Zhou W,Zhu B,Zeng Y,Yao K,Ren C

更新日期：2008-09-01 00:00:00

abstract：:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...

journal_title：Molecular biotechnology

authors： Gach JS,Quendler H,Ferko B,Katinger H,Kunert R

更新日期：2008-06-01 00:00:00

abstract：:ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...

journal_title：Molecular biotechnology

authors： Wu HJ,Luo J,Wu N,Matand K,Zhang LJ,Han XF,Yang BJ

更新日期：2008-05-01 00:00:00

abstract：:A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...

journal_title：Molecular biotechnology

authors： Mabrouk SB,Messaoud EB,Ayadi D,Jemli S,Roy A,Mezghani M,Bejar S

更新日期：2008-03-01 00:00:00

abstract：:A new lipase gene designated as SlLipA was isolated from Serratia liquefaciens S33 DB-1 by the genomic-walking method. The cloned gene contained an open reading frame (ORF) of 1,845 bp encoding 615 amino acids with a conserved GXSXG motif. Genome sequence analysis showed that an aldo/keto reductase gene closed to the ...

journal_title：Molecular biotechnology

authors： Yao H,Yu S,Zhang L,Zuo K,Ling H,Zhang F,Tang K

更新日期：2008-02-01 00:00:00

abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...

journal_title：Molecular biotechnology

authors： Wang X,Tian W,Li Y

更新日期：2008-01-01 00:00:00

abstract：:De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...

journal_title：Molecular biotechnology

authors： Dean PM

更新日期：2007-11-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light...

journal_title：Molecular biotechnology

authors： Stahl W,Sies H

更新日期：2007-09-01 00:00:00

abstract：:The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...

journal_title：Molecular biotechnology

authors： Russell RB

更新日期：2007-07-01 00:00:00

abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...

journal_title：Molecular biotechnology

authors： Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul S

更新日期：2007-06-01 00:00:00

abstract：:A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of ...

journal_title：Molecular biotechnology

authors： Canto-Canché B,Guillén-Maldonado DK,Peraza-Echeverría L,Conde-Ferráez L,James-Kay A

更新日期：2007-05-01 00:00:00

abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...

journal_title：Molecular biotechnology

authors： Gall JG,Lizonova A,EttyReddy D,McVey D,Zuber M,Kovesdi I,Aughtman B,King CR,Brough DE

更新日期：2007-03-01 00:00:00

abstract：:Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...

journal_title：Molecular biotechnology

authors： Rouis S,Chakroun M,Saadaoui I,Jaoua S

更新日期：2007-02-01 00:00:00

abstract：:The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...

journal_title：Molecular biotechnology

authors： Phillips C

更新日期：2007-01-01 00:00:00

abstract：:Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy...

journal_title：Molecular biotechnology

authors： Liu YT,Kardosh A,Cooc J,Schönthal AH

更新日期：2006-11-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...

journal_title：Molecular biotechnology

authors： Perreau M,Kremer EJ

更新日期：2006-10-01 00:00:00

abstract：:High-quality RNA preparations are critical for further applications such as reverse transcriptase-polymerase chain reaction (RT-PCR) transcript amplifications, and elaboration of cDNA and expressed sequence tag libraries. Melanins are phenolic compounds present in many fungi and apparently play key roles in fungi path...

journal_title：Molecular biotechnology

authors： Islas-Flores I,Peraza-Echeverría L,Canto-Canché B,Rodríguez-García CM

更新日期：2006-09-01 00:00:00

abstract：:The implementation of efficient technologies for the production of recombinant mammalian membrane receptors is an outstanding challenge in understanding receptor-ligand actions and the development of therapeutic antibodies. In order to improve the solubility of recombinant extracellular domains of human membrane recep...

journal_title：Molecular biotechnology

authors： Endo S,Tomimoto Y,Shimizu H,Taniguchi Y,Onizuka T

更新日期：2006-07-01 00:00:00

abstract：:A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...

journal_title：Molecular biotechnology

authors： Gargouri-Bouzid R,Jaoua L,Rouis S,Saïdi MN,Bouaziz D,Ellouz R

更新日期：2006-06-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...

journal_title：Molecular biotechnology

authors： Kang TJ,Kim BG,Yang JY,Yang MS

更新日期：2006-02-01 00:00:00

abstract：:Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...

journal_title：Molecular biotechnology

authors： Karaman S,Cunnick J,Wang K

更新日期：2006-01-01 00:00:00

abstract：:Many preclinical studies have shown RNA interference (RNAi) as a new promising way to treat various human diseases including cancer and virus infection and there is an increasing demand for the large-scale preparation of short interfering RNAs (siRNAs) at low cost. Data are accumulating to show that endoribonuclease-p...

journal_title：Molecular biotechnology

authors： Xuan B,Qian Z,Tan C,Min T,Shen S,Huang W

更新日期：2005-11-01 00:00:00

abstract：:An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase wi...

journal_title：Molecular biotechnology

authors： Ruiz-Sanchez A,Cruz-Camarillo R,Salcedo-Hernandez R,Ibarra JE,Barboza-Corona JE

更新日期：2005-10-01 00:00:00

abstract：:The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to ...

journal_title：Molecular biotechnology

authors： Lee YJ,Keng PC

更新日期：2005-09-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor, that has been used as a therapeutic agent in facilitating bone marrow and stem cell transplantation and in other clinical cases like neutropenia. Although biologically active recombinant GM-CSF has been successfully produced in...

journal_title：Molecular biotechnology

authors： Bhattacharya P,Pandey G,Srivastava P,Mukherjee KJ

更新日期：2005-06-01 00:00:00

abstract：:A single-stage polymerase-based procedure is described that allows extensive modifications of DNA. The version described here uses the QuikChange Site-Directed Mutagenesis System kit supplied by Stratagene. The original protocol is replaced by a single-stage method in which linear production of complementary strands i...

journal_title：Molecular biotechnology

authors： Salerno JC,Jones RJ,Erdogan E,Smith SM

更新日期：2005-03-01 00:00:00

abstract：:Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...

journal_title：Molecular biotechnology

authors： Ristevski S

更新日期：2005-02-01 00:00:00

abstract：:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...

journal_title：Molecular biotechnology

authors： Chuderland D,Seger R

更新日期：2005-01-01 00:00:00

abstract：:RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especiall...

journal_title：Molecular biotechnology

authors： Zhao N,Zu ZX,Liu CM,Dong WJ,Liu DP,Liang CC

更新日期：2004-11-01 00:00:00

abstract：:Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites ...

journal_title：Molecular biotechnology

authors： Santosa DA,Hendroko R,Farouk A,Greiner R

更新日期：2004-10-01 00:00:00

abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...

journal_title：Molecular biotechnology

authors： Djurovic S,Iversen N,Jeansson S,Hoover F,Christensen G

更新日期：2004-09-01 00:00:00

abstract：:In mammals, CpG methylation is one of the mechanisms of epigenetic control over the linear sequence of bases of deoxyribonucleic acid (DNA); about 70% of CpG dinucleotides are methylated. The actual signal that triggers DNA methylation is not known, although repetitive DNA has been shown to be an attractive template f...

journal_title：Molecular biotechnology

authors： Pena RN,Webster J,Kwan S,Korbel J,Whitelaw BA

更新日期：2004-03-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field ...

journal_title：Molecular biotechnology

authors： Jurinke C,Oeth P,van den Boom D

更新日期：2004-02-01 00:00:00